in-B701 Throughput 12 Automatic PCR Machine Nucleic Acid Extraction System DNA Extractor

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Description:

 

In ultrasonic extraction, mechanical energy generated by ultrasonic waves is applied to the sample (as shown in the figure below). This sonication results in cavitation, the creation of small vacuum bubbles or voids in the liquid that burst onto the solid sample, resulting in localized high temperatures (~4500°C) and pressure (~50 MPa). These forces produce effects such as sonication, disruption of cell membranes and extraction of intracellular material.

1. Application in nicotine extraction

The nicotine component in tobacco has high use value in agriculture and medicine, and the nicotine component in tobacco can be effectively extracted by using ultrasonic extraction technology. In this aspect of application research, the researchers mainly investigated the influence of factors such as extractant concentration, solid-liquid ratio, extraction time, and ultrasonic temperature on the extraction efficiency of nicotine components.  40% methanol as the extractant, the solid-liquid ratio was 1:20, the extraction time was 30 minutes, the ultrasonic temperature was 150°C, and the extraction rate of nicotine could reach about 7%. Chu Zhibing and others used 0.4% NaOH as the extractant, the solid-liquid ratio was 1:40, and the ultrasonic extraction time at room temperature was 4 hours, and the extraction rate of nicotine was 2.12%. 78% ethanol was used as the extractant, the solid-liquid ratio was 1:5, the extraction time was 30min, and the ultrasonic temperature was 52°C.

The extraction amount of nicotine is 23.1mg/mL. Using 95% ethanol with pH = 4 as solvent, solid-liquid ratio 1:15, ultrasonic temperature 80 ℃ extraction twice, each 45min, nicotine extraction rate can reach 95.42%, comparative analysis shows that its extraction effect is obviously better than heating Reflux extraction (the latter is only 84.79%).

2. Application in extraction of total flavonoids from lotus seed heart

Taking lotus seed heart as the test material and ethanol solution as the extractant, ultrasonic extraction method was used to conduct single factor test and L9(34) orthogonal test to study the effects of ethanol concentration, extraction time, solid-liquid ratio and extraction temperature on the extraction rate of total flavonoids. Influence. The results show that the main factor affecting the extraction rate of total flavonoids in lotus seeds is the concentration of ethanol, followed by extraction temperature, solid-liquid ratio, and ultrasonic extraction time; the optimal conditions for extraction are: ethanol concentration 60%, extraction temperature 70°C, Ratio 1:24g/mL, ultrasonic extraction time 30min, the extraction rate of total flavonoids obtained under this condition is 10.86mg/g.

3. Application in Lycium barbarum polysaccharide extraction

The extraction process of Lycium barbarum polysaccharides was extracted by ultrasonic wave, the content of Lycium barbarum polysaccharides was measured by spectrophotometric colorimetry, and the extraction process of Lycium barbarum polysaccharides by ultrasonic-assisted water leaching was systematically studied through the stop-cross experiment, and the optimal process was determined, namely: at 50 ℃, material-water ratio of 1:60, soaking for 2.5 hours, ultrasonic extraction for 5 minutes, the highest extraction rate of polysaccharides was 50.36%.

Parameter:

Model/Data	Sono-20-1000	Sono-20-2000	Sono-20-3000	Sono-15-3000
Frequency	20±0.5 KHz	20±0.5 KHz	20±0.5 KHz	15±0.5 KHz
Power	1000W	2000W	3000W	3000W
Voltage	110/220V
Temperature	300℃
Pressure	35 MPa
Intensity of sound	20 W/cm²	40 W/cm²	60 W/cm²	60 W/cm²
Max Capacity	10 L/Min	15 L/Min	20 L/Min	20 L/Min
Horn Material	Titanium

 

Advantages

 

1) No need for high temperature. Ultrasonic intensified extraction at 40℃-50℃ water temperature F, ultrasound can promote plants

2) Atmospheric extraction, good safety, simple operation and convenient maintenance.

3) High extraction efficiency. The best extraction rate can be obtained after 20-40 minutes of ultrasonic enhanced extraction, basically

4) It has a broad spectrum. The applicability is wide, and most of the various components of Chinese medicinal materials can be extracted by ultrasonic.

5) Ultrasonic extraction has little relationship with the properties of the solvent and the target extract. Therefore, there are many types of extraction solvents to choose from and a wide range of target extracts.

6) Reduce energy consumption. Because ultrasonic extraction does not require heating or the heating temperature is low, the extraction time is short, so energy consumption is greatly reduced.

7) The processing capacity of medicinal raw materials is large, doubled or several times higher, and there are few impurities, and the effective ingredients are easy to separate and purify.

8) The extraction process has low cost and significant comprehensive economic benefits.

 

For us, providing better in-B701 Throughput 12 Automatic PCR Machine Nucleic Acid Extraction System DNA Extractor in terms of quality and design is our only way out in the market competition. We adhere to the business philosophy of "customer-centric, customer-centric" and dedicated to providing high-quality products to Chinese and foreign customers. The company has offices all over the country with professional, high-quality, and strong communication skills after-sales service personnel to provide you with high-quality products and efficient services.

Hot Tags: industrial ultrasound equipment, China, suppliers, manufacturers, factory, custom, sonicator instrument, Mushroom extraction process, emulsifier blender, Ultrasonic liquid processor, ultrasonic emulsifying machine, ultrasonic solvent extraction

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