Specific Steps For Extracting Chlorophyll Through Cell Fragmentation Using An Ultrasonic Cell Crusher

Ultrasonic cell crusher utilizes the dispersion effect of ultrasound in liquid to create cavitation, thereby breaking solid particles or cell tissues in the liquid.
The development of the biological industry has raised the requirements for experiments using ultrasonic cell pulverizers, such as measuring and controlling sample temperature, improving the intelligence level of low-temperature cooling samples and the entire machine, and so on.
The specific steps for extracting chlorophyll through ultrasonic cell crushing using an ultrasonic cell crusher:
1. Centrifuge or filter the water sample and install an acetate fiber filter membrane on the suction filter. Pour a quantitative volume of water sample for filtration, and the negative pressure during filtration should not be too high (about 50kPa). After the water sample is drawn, continue to draw for 1-2 minutes to reduce the moisture on the filter membrane. If short-term storage is required for 1-2 days, it can be stored in a regular refrigerator for freezing. If long-term storage is required (30 days), it should be stored in a low-temperature refrigerator (-20 ℃).
2. Take out the filter membrane containing phytoplankton and dry it at low temperature in the refrigerator for 6-8 hours. Cut it into small pieces with scissors and place it in a 5ml or 10ml centrifuge tube. Add 3-4 ml of 90% acetone to immerse the fragments below the liquid level. Place the fragments in an ultrasonic container and use ultrasonic waves to break the cells of the sample. (Comparing experiments at different times and frequencies to facilitate the identification of * fragmentation time and frequency for unified methods).
3. Centrifuge the sample subjected to cell fragmentation using ultrasound using a centrifuge (3000-4000r/min) for 10 minutes. Pour the supernatant into a 5ml or 10ml volumetric flask. Dilute to 5ml or 10ml with 90% acetone and shake well.
4. Place the supernatant on a spectrophotometer and use a 1cm optical path colorimetric dish to read the absorbance values at wavelengths of 750nm, 663nm, 645nm, and 630nm, respectively. Use 90% acetone as a blank absorbance measurement to correct the absorbance of the sample.